Our papers


Arranged thematically below - or find us on PubMed or GoogleScholar.
(meta)effectors  |   CRISPR-Cas   | pathogen evolution



Valleau D, Quaile AT, Cui H, Xu X, Evdokimova E, Chang C, Cuff ME, Urbanus ML,
Houliston S, Arrowsmith CH, Ensminger AW, Savchenko A. Discovery of Ubiquitin
Deamidases in the Pathogenic Arsenal of Legionella pneumophila. Cell Rep. 2018
Apr 10;23(2):568-583. doi: 10.1016/j.celrep.2018.03.060. PubMed PMID: 29642013.

Urbanus ML, Quaile AT, Stogios PJ, Morar M, Rao C, Di Leo R, Evdokimova E, Lam
M, Oatway C, Cuff ME, Osipiuk J, Michalska K, Nocek BP, Taipale M, Savchenko A,
Ensminger AW. Diverse mechanisms of metaeffector activity in an intracellular
bacterial pathogen, Legionella pneumophila. Mol Syst Biol. 2016 Dec
16;12(12):893. doi: 10.15252/msb.20167381. PubMed PMID: 27986836.

Ensminger AW. Legionella pneumophila, armed to the hilt: justifying the
largest arsenal of effectors in the bacterial world. Curr Opin Microbiol. 2016
Feb;29:74-80. doi: 10.1016/j.mib.2015.11.002. Epub 2015 Dec 19. Review. PubMed
PMID: 26709975.

Quaile AT, Urbanus ML, Stogios PJ, Nocek B, Skarina T, Ensminger AW, Savchenko
A. Molecular Characterization of LubX: Functional Divergence of the U-Box Fold by
Legionella pneumophila. Structure. 2015 Aug 4;23(8):1459-1469. doi:
10.1016/j.str.2015.05.020. Epub 2015 Jul 2. PubMed PMID: 26146184.

Quaile AT, Stogios PJ, Egorova O, Evdokimova E, Valleau D, Nocek B, Kompella
PS, Peisajovich S, Yakunin AF, Ensminger AW, Savchenko A. The Legionella
pneumophila effector Ceg4 is a phosphotyrosine phosphatase that attenuates
activation of eukaryotic MAPK pathways. J Biol Chem. 2018 Mar 2;293(9):3307-3320.
doi: 10.1074/jbc.M117.812727. Epub 2018 Jan 4. PubMed PMID: 29301934.

Morar M, Evdokimova E, Chang C, Ensminger AW, Savchenko A. Crystal structure
of the Legionella pneumophila Lem10 effector reveals a new member of the HD
protein superfamily. Proteins. 2015 Dec;83(12):2319-25. doi: 10.1002/prot.24933. 
Epub 2015 Oct 14. PubMed PMID: 26426142.

Ensminger AW, Isberg RR. E3 ubiquitin ligase activity and targeting of BAT3 by
multiple Legionella pneumophila translocated substrates. Infect Immun. 2010
Sep;78(9):3905-19. doi: 10.1128/IAI.00344-10. Epub 2010 Jun 14. PubMed PMID:

Ensminger AW, Isberg RR. Legionella pneumophila Dot/Icm translocated
substrates: a sum of parts. Curr Opin Microbiol. 2009 Feb;12(1):67-73. doi:
10.1016/j.mib.2008.12.004. Epub 2009 Jan 20. Review. PubMed PMID: 19157961.



Deecker SR, Ensminger AW. Working together: cross-priming in two Legionella pneumophila type I-F CRISPR-Cas systems. bioRxiv 276188; doi: https://doi.org/10.1101/276188 

Rao C, Chin D, Ensminger AW. Priming in a permissive type I-C CRISPR-Cas
system reveals distinct dynamics of spacer acquisition and loss. RNA. 2017
Oct;23(10):1525-1538. doi: 10.1261/rna.062083.117. Epub 2017 Jul 19. PubMed PMID: 28724535.

Rao C, Guyard C, Pelaz C, Wasserscheid J, Bondy-Denomy J, Dewar K, Ensminger
AW. Active and adaptive Legionella CRISPR-Cas reveals a recurrent challenge to
the pathogen. Cell Microbiol. 2016 Oct;18(10):1319-38. doi: 10.1111/cmi.12586.
Epub 2016 Mar 31. PubMed PMID: 26936325.




Ali SS, Soo J, Rao C, Leung AS, Ngai DH, Ensminger AW, Navarre WW. Silencing
by H-NS potentiated the evolution of Salmonella. PLoS Pathog. 2014 Nov
6;10(11):e1004500. doi: 10.1371/journal.ppat.1004500. eCollection 2014 Nov.
PubMed PMID: 25375226.

Ensminger AW. Experimental evolution of pathogenesis: "patient" research. PLoS
Pathog. 2013;9(5):e1003340. doi: 10.1371/journal.ppat.1003340. Epub 2013 May 30. 
Review. PubMed PMID: 23737744.

Rao C, Benhabib H, Ensminger AW. Phylogenetic reconstruction of the Legionella
pneumophila Philadelphia-1 laboratory strains through comparative genomics. PLoS 
One. 2013 May 22;8(5):e64129. doi: 10.1371/journal.pone.0064129. Print 2013.
PubMed PMID: 23717549.

Ensminger AW, Yassin Y, Miron A, Isberg RR. Experimental evolution of
Legionella pneumophila in mouse macrophages leads to strains with altered
determinants of environmental survival. PLoS Pathog. 2012;8(5):e1002731. doi:
10.1371/journal.ppat.1002731. Epub 2012 May 31. PubMed PMID: 22693450.